Journal: The Journal of Neuroscience

Article Title: Nerve Growth Factor Treatment Increases Brain-Derived Neurotrophic Factor Selectively in TrkA-Expressing Dorsal Root Ganglion Cells and in Their Central Terminations within the Spinal Cord

doi: 10.1523/JNEUROSCI.17-21-08476.1997

Figure Lengend Snippet: BDNF mRNA and protein are increased by NGF treatment. BDNF immunofluorescence (a, c, e) andin situ hybridization (b, d, f) in lumbar ganglia of control (a, b), intraperitoneal NGF-treated (c, d), and intrathecal NGF-treated (e, f) rats. BDNF immunoreactivity is present in small to medium sized DRG cells and is increased after NGF treatment. The increase is most evident after intrathecal NGF (e), where immunoreactivity is seen not only in a larger number of DRG cells but also in neighboring axons (arrows). NGF treatment also increases expression of BDNF mRNA. In control tissue (b), a few heavily labeled cells are seen (stars) together with scattered light labeling (arrows). The number of heavily labeled cells is increased after intraperitoneal NGF (d) and increased even more by intrathecal NGF (f). Scale bars, 100 μm.

Article Snippet: Sections were stained using standard single or dual color indirect labeled immunofluorescence or indirect tyramide signal amplification (TSA) (NEN) fluorescence procedures ( Priestley, 1997 ).

Techniques: Immunofluorescence, Hybridization, Control, Expressing, Labeling

Journal: The Journal of Neuroscience

Article Title: Nerve Growth Factor Treatment Increases Brain-Derived Neurotrophic Factor Selectively in TrkA-Expressing Dorsal Root Ganglion Cells and in Their Central Terminations within the Spinal Cord

doi: 10.1523/JNEUROSCI.17-21-08476.1997

Figure Lengend Snippet: BDNF immunoreactivity is present in trkA/CGRP cells and is increased by NGF treatment. Double-labeling for BDNF (a, c, e) and either trkA (b, d) or CGRP (f) immunofluorescence in lumbar ganglia of control (a, b) and intrathecal NGF-treated (c–f) rats is shown. In both control and intrathecally treated animals, the majority of BDNF-immunoreactive DRG cells are also trkA immunoreactive (a–d). However, because intrathecal NGF increases the level of BDNF immunoreactivity, the number of trkA-immunoreactive cells that double-label for BDNF is increased in c and d compared witha and b. A similar situation occurs for BDNF and CGRP double-labeling, with extensive coexistence of BDNF and CGRP evident after intrathecal NGF treatment (e, f). Arrows indicate BDNF/trkA or BDNF/CGRP double-labeled cells; asterisks indicate cells single-labeled for trkA or CGRP; stars indicate cells single-labeled for BDNF. Scale bars, 100 μm.

Article Snippet: Sections were stained using standard single or dual color indirect labeled immunofluorescence or indirect tyramide signal amplification (TSA) (NEN) fluorescence procedures ( Priestley, 1997 ).

Techniques: Labeling, Immunofluorescence, Control

Journal: The Journal of Neuroscience

Article Title: Nerve Growth Factor Treatment Increases Brain-Derived Neurotrophic Factor Selectively in TrkA-Expressing Dorsal Root Ganglion Cells and in Their Central Terminations within the Spinal Cord

doi: 10.1523/JNEUROSCI.17-21-08476.1997

Figure Lengend Snippet: BDNF immunoreactivity in the spinal cord is increased by NGF treatment and is present in CGRP-immunoreactive axons.a–d show BDNF immunofluorescence in the dorsal horn of control tissue (a, b) and after intrathecal NGF treatment (c, d). BDNF immunoreactivity in laminae I and II (asterisks in a and c) and in deep dorsal horn (arrowheads in c) is increased by NGF. The increase is particularly striking in lateral lamina II (stars in a andc), and this region is shown at high magnification inb and d. Immunoreactive axons (arrows in b and d) are more abundant after NGF treatment. e–h, Double-labeling showing extensive coexistence of BDNF (e, g) and CGRP (f, h) after intrathecal NGF treatment. In the dorsal horn (e, f) and in lamina X dorsolateral to the aqueduct (g, h), numerous double-labeled axons and varicosities (arrows) are visible. Scale bars:a, c, 100 μm; b, d, 25 μm;e–h, 50 μm.

Article Snippet: Sections were stained using standard single or dual color indirect labeled immunofluorescence or indirect tyramide signal amplification (TSA) (NEN) fluorescence procedures ( Priestley, 1997 ).

Techniques: Immunofluorescence, Control, Labeling